Dung from a mammoth was preserved under frozen conditions in Alaska. The mammoth lived during the
early part of the Late Glacial interstadial (ca 12,300 BP). Microfossils, macroremains and ancient DNA
from the dung were studied and the chemical composition was determined to reconstruct both the
paleoenvironment and paleobiology of this mammoth. Pollen spectra are dominated by Poaceae, Artemisia
and other light-demanding taxa, indicating an open, treeless landscape (‘mammoth steppe’). Fruits
and seeds support this conclusion. The dung consists mainly of cyperaceous stems and leaves, with
a minor component of vegetative remains of Poaceae. Analyses of fragments of the plastid rbcL gene and
trnL intron and nrITS1 region, amplified from DNA extracted from the dung, supplemented the microscopic
identifications. Many fruit bodies with ascospores of the coprophilous fungus Podospora conica
were found inside the dung ball, indicating that the mammoth had eaten dung. The absence of bile acids
points to mammoth dung. This is the second time that evidence for coprophagy of mammoths has been
derived from the presence of fruit bodies of coprophilous fungi in frozen dung. Coprophagy might well
have been a common habit of mammoths. Therefore, we strongly recommend that particular attention
should be given to fungal remains in future fossil dung studies.